Third-generation sequencing found LncRNA associated with heat shock protein response to heat stress in Populus qiongdaoensis seedlings.
Identifieur interne : 000052 ( Main/Exploration ); précédent : 000051; suivant : 000053Third-generation sequencing found LncRNA associated with heat shock protein response to heat stress in Populus qiongdaoensis seedlings.
Auteurs : Jiahong Xu [République populaire de Chine] ; Yao Zheng [République populaire de Chine] ; Shouqin Pu [République populaire de Chine] ; Xiujie Zhang [République populaire de Chine] ; Zhihao Li [République populaire de Chine] ; Jinhui Chen [République populaire de Chine]Source :
- BMC genomics [ 1471-2164 ] ; 2020.
Abstract
BACKGROUND
As air temperatures increase globally, more and more plants are exposed to heat-stress conditions. Although many studies have explored regulation networks in plants with the aim of improving their heat-stress tolerance, only few have revealed them in trees. Here, individuals of Populus qiongdaoensis seedlings, which grows naturally in tropical areas, exposed to heat at 40 °C and the non-coding regulation networks were explored using the PacBio RSII and the Illumina sequencing platform.
RESULTS
In total, we obtained 88,161 full-length transcripts representing 39,343 genes using 5,498,988 long reads and 350,026,252 clean reads, and also 216 microRNAs (miRNAs) via 95,794,107 reads. We then identified 928 putative long non-coding RNAs (lncRNAs), consisting of 828 sense lncRNAs (89.22%), 34 long intergenic non-coding RNAs (3.66%), 16 antisense (1.72%), and 50 sense intronic lncRNAs (5.39%). Under the dual criteria of |log
CONCLUSIONS
Functional enrichment analysis of target genes of miRNAs and lncRNAs indicated that miRNAs and lncRNAs play an important role in the response to heat stress P. qiongdaoensis. Lastly, by investigating the miRNA-lncRNA-mRNA network of this species, we revealed that miRNAs may negatively regulate both lncRNAs and mRNAs in tree responses to heat stress, and found that lncHSP18.2 may cis-regulate HSP18.2.
DOI: 10.1186/s12864-020-06979-z
PubMed: 32831021
PubMed Central: PMC7444055
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiujie" sort="Zhang, Xiujie" uniqKey="Zhang X" first="Xiujie" last="Zhang">Xiujie Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Li, Zhihao" sort="Li, Zhihao" uniqKey="Li Z" first="Zhihao" last="Li">Zhihao Li</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Jinhui" sort="Chen, Jinhui" uniqKey="Chen J" first="Jinhui" last="Chen">Jinhui Chen</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China. jinhuichen@hainanu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China. jinhuichen@hainanu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32831021</idno><idno type="pmid">32831021</idno><idno type="doi">10.1186/s12864-020-06979-z</idno><idno type="pmc">PMC7444055</idno><idno type="wicri:Area/Main/Corpus">000127</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000127</idno><idno type="wicri:Area/Main/Curation">000127</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000127</idno><idno type="wicri:Area/Main/Exploration">000127</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Third-generation sequencing found LncRNA associated with heat shock protein response to heat stress in Populus qiongdaoensis seedlings.</title><author><name sortKey="Xu, Jiahong" sort="Xu, Jiahong" uniqKey="Xu J" first="Jiahong" last="Xu">Jiahong Xu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Yao" sort="Zheng, Yao" uniqKey="Zheng Y" first="Yao" last="Zheng">Yao Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Pu, Shouqin" sort="Pu, Shouqin" uniqKey="Pu S" first="Shouqin" last="Pu">Shouqin Pu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiujie" sort="Zhang, Xiujie" uniqKey="Zhang X" first="Xiujie" last="Zhang">Xiujie Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Li, Zhihao" sort="Li, Zhihao" uniqKey="Li Z" first="Zhihao" last="Li">Zhihao Li</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Jinhui" sort="Chen, Jinhui" uniqKey="Chen J" first="Jinhui" last="Chen">Jinhui Chen</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China. jinhuichen@hainanu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation><affiliation wicri:level="1"><nlm:affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China. jinhuichen@hainanu.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country></affiliation></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>As air temperatures increase globally, more and more plants are exposed to heat-stress conditions. Although many studies have explored regulation networks in plants with the aim of improving their heat-stress tolerance, only few have revealed them in trees. Here, individuals of Populus qiongdaoensis seedlings, which grows naturally in tropical areas, exposed to heat at 40 °C and the non-coding regulation networks were explored using the PacBio RSII and the Illumina sequencing platform.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In total, we obtained 88,161 full-length transcripts representing 39,343 genes using 5,498,988 long reads and 350,026,252 clean reads, and also 216 microRNAs (miRNAs) via 95,794,107 reads. We then identified 928 putative long non-coding RNAs (lncRNAs), consisting of 828 sense lncRNAs (89.22%), 34 long intergenic non-coding RNAs (3.66%), 16 antisense (1.72%), and 50 sense intronic lncRNAs (5.39%). Under the dual criteria of |log</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Functional enrichment analysis of target genes of miRNAs and lncRNAs indicated that miRNAs and lncRNAs play an important role in the response to heat stress P. qiongdaoensis. Lastly, by investigating the miRNA-lncRNA-mRNA network of this species, we revealed that miRNAs may negatively regulate both lncRNAs and mRNAs in tree responses to heat stress, and found that lncHSP18.2 may cis-regulate HSP18.2.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32831021</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>27</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Aug</Month><Day>24</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Third-generation sequencing found LncRNA associated with heat shock protein response to heat stress in Populus qiongdaoensis seedlings.</ArticleTitle><Pagination><MedlinePgn>572</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12864-020-06979-z</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">As air temperatures increase globally, more and more plants are exposed to heat-stress conditions. Although many studies have explored regulation networks in plants with the aim of improving their heat-stress tolerance, only few have revealed them in trees. Here, individuals of Populus qiongdaoensis seedlings, which grows naturally in tropical areas, exposed to heat at 40 °C and the non-coding regulation networks were explored using the PacBio RSII and the Illumina sequencing platform.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In total, we obtained 88,161 full-length transcripts representing 39,343 genes using 5,498,988 long reads and 350,026,252 clean reads, and also 216 microRNAs (miRNAs) via 95,794,107 reads. We then identified 928 putative long non-coding RNAs (lncRNAs), consisting of 828 sense lncRNAs (89.22%), 34 long intergenic non-coding RNAs (3.66%), 16 antisense (1.72%), and 50 sense intronic lncRNAs (5.39%). Under the dual criteria of |log<sub>2</sub>fold-change| ≥ 1 and P-value < 0.05, 1690 genes, 25 lncRNAs, and 15 miRNAs were found differentially expressed under the heat stress treatment. Furthermore, 563 and 595 mRNAs were detected as target genes of 14 differently expressed miRNAs and 26 differentially expressed lncRNAs. Functional annotation analysis of these target genes demonstrated they were related to cell membrane stability, plant hormone signal transduction, antioxidation, and aldarate metabolism. Lastly, we uncovered a key interaction network of lncRNAs, miRNAs and mRNAs that consisted of miR1444d, miR482a.1, miR530a, lncHSP18.2, HSP18.1, and HSP18.2. Expression level analysis showed that miRNAs in the network were up-regulated, while mRNAs and lncRNA were down-regulated, and also found that lncHSP18.2 may cis-regulate HSP18.2.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Functional enrichment analysis of target genes of miRNAs and lncRNAs indicated that miRNAs and lncRNAs play an important role in the response to heat stress P. qiongdaoensis. Lastly, by investigating the miRNA-lncRNA-mRNA network of this species, we revealed that miRNAs may negatively regulate both lncRNAs and mRNAs in tree responses to heat stress, and found that lncHSP18.2 may cis-regulate HSP18.2.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Jiahong</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Yao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pu</LastName><ForeName>Shouqin</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xiujie</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zhihao</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jinhui</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-9619-0539</Identifier><AffiliationInfo><Affiliation>Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education / Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, 570228, P. R. China. jinhuichen@hainanu.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, Institute of Tropical Agriculture and Forestry, College of Forestry, Hainan University, Haikou, 570228, P. R. China. jinhuichen@hainanu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>318MS009</GrantID><Agency>Hainan Provincial Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>K2018201</GrantID><Agency>Opening Project of State Key Laboratory of Tree Genetics and Breeding</Agency><Country></Country></Grant><Grant><GrantID>KYQD(ZR)1830</GrantID><Agency>Scientific Research Fund Project of Hainan University</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList 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